Motor Function in α-Synuclein Mice Across Gut Microbiome Treatments

Motor function tests (beam, pole, adhesive removal, hindlimb clasping) comparing wild-type and α-synuclein overexpressing mice across 5 microbiome conditions: germ-free, antibiotics, SPF, ex-germ-free, and SCFA (n=105).
# Treatment
Genotype
Avg Beam cross time (sec)
Avg Pole descent time (sec)
Avg Adhesive removal time (sec)
Hindlimb score
+
1 SPF WT 6.32 4.87 2.29 0
2 SPF WT 4.79 4.33 5.58 0
3 SPF WT 4.23 6.25 5.18 0
4 SPF WT 5.37 7.44 3.8 0
5 SPF WT 4.89 4.77 1.58 1
6 SPF WT 5.29 6.64 1.31 0
7 SPF WT 5.8 6.86 1.56 nd
8 SPF WT 4.44 8.47 3.42 nd
9 SPF WT 3.51 4.92 2.53 nd
10 SPF WT 3.54 7.25 2.44 nd
11 SPF ASO 8.99 4.58 12.16 2
12 SPF ASO 8.93 8.48 2.38 1
13 SPF ASO 6.86 13.63 3.38 1
14 SPF ASO 6.36 9.34 1.41 1
15 SPF ASO 9 25.57 2.86 2
16 SPF ASO 8.24 9.19 7.36 nd
17 SPF ASO 5.31 14.59 2.19 nd
18 SPF ASO 9.06 18.97 11 nd
19 SPF ASO 9.43 12.08 22.28 nd
20 SPF ASO 7.12 6.32 16.78 nd
21 GF WT 6.676 4.89 3.56 0
22 GF WT 6.125 4.93 1.88 0
23 GF WT 4.895 2.18 3.53 0
24 GF WT 4.99 4.29 8.29 0
25 GF WT 9.92 3.01 1.26 0
26 GF WT 7.63 4.06 1.19 1
27 GF WT 1.98 8.6 1.14 nd
28 GF WT 2.62 6.09 3.22 nd
29 GF WT 6 2.18 1.81 nd
30 GF WT 3.71 4.29 3.03 nd
31 GF WT 3.04 3.01 4.78 nd
32 GF ASO 5.18 6.68 5.22 0
33 GF ASO 6.97 3.91 1.42 1
34 GF ASO 6.06 4.42 7.47 0
35 GF ASO 5.46 3.66 3.95 0
36 GF ASO 5.73 9.93 9.81 1
37 GF ASO 7.53 4.06 1.76 1
38 GF ASO 5.16 4.97 5 nd
39 GF ASO 3.82 3.49 6.47 nd
40 GF ASO 5.62 3.8 2.08 nd
41 GF ASO 3 8.65 5.13 nd
42 GF ASO 3.87 12.77 3.65 nd
43 Abx WT 2.56 2.91 2.42 1
44 Abx WT 4.06 9.26 1.2 0
45 Abx WT 8.18 5.79 15.56 0
46 Abx WT 7.68 5.38 5.63 0
47 Abx WT 4.81 4.62 3.64 0
48 Abx WT 5.47 5.58 9.52 0
49 Abx WT 6.32 11.96 7.12 0
50 Abx WT 6.82 5.94 6.01 nd
51 Abx WT 5.59 7.01 3.42 nd
52 Abx WT 4.3 7.38 nd nd
53 Abx WT 3.94 3.84 nd nd
54 Abx WT 4.04 6.96 nd nd
55 Abx WT 4.07 7.52 nd nd
56 Abx WT 3.24 5.96 nd nd
57 Abx WT 2.82 5.96 nd nd
58 Abx ASO 5.19 6.91 8.62 1
59 Abx ASO 4.64 7.49 3.36 1
60 Abx ASO 4.66 4.22 14.59 1
61 Abx ASO 6.07 5.49 6.24 0
62 Abx ASO 4.69 8.7 14.42 nd
63 Abx ASO 5.97 4.81 5.4 nd
64 Abx ASO 7.02 4.35 9.19 nd
65 Abx ASO 4.27 6.65 4.81 nd
66 Abx ASO 3.77 7.25 6.87 nd
67 Abx ASO 4 8.95 1.51 nd
68 Abx ASO 3.65 7.27 1.92 nd
69 ExGF WT 4.24 4.01 1.74 0
70 ExGF WT 4.63 6.53 3.19 0
71 ExGF WT 4.41 3.02 1.55 0
72 ExGF WT 4.84 5.16 3.15 0
73 ExGF WT 4.55 4.33 5.36 nd
74 ExGF WT 4.39 8.25 1.31 nd
75 ExGF WT 4.56 2.02 1.56 nd
76 ExGF WT 3.59 2.59 3.42 nd
77 ExGF WT 2.99 5.65 2.53 nd
78 ExGF WT 2.76 4.93 2.44 nd
79 ExGF ASO 8.49 18.31 11.72 1
80 ExGF ASO 9.18 7.39 2.7 1
81 ExGF ASO 5.27 9.27 21.26 1
82 ExGF ASO 6.49 10.04 1.63 1
83 ExGF ASO 8.07 4.63 2.4 2
84 ExGF ASO 8.17 9.29 8.18 nd
85 ExGF ASO 6.89 8.97 20.72 nd
86 ExGF ASO 6.82 8.24 4.52 nd
87 ExGF ASO 5.61 22.67 9.81 nd
88 ExGF ASO 6.26 6.87 7.36 nd
89 ExGF ASO 5.88 5.81 2.19 nd
90 ExGF ASO 4.54 5.37 11 nd
91 ExGF ASO 4.71 5.64 22.28 nd
92 ExGF ASO 8.06 8.67 16.78 nd
93 SCFA WT 8.51 4.84 1.79 0
94 SCFA WT 4.65 9.51 1.46 0
95 SCFA WT 4.89 3.68 1.4 0
96 SCFA WT 4.14 4.27 3.635 0
97 SCFA WT 3.23 7.60 1.55 0
98 SCFA WT 4.31 4.61 2.15 1
99 SCFA ASO 5.3 13.86 3.49 1
100 SCFA ASO 8.49 11.26 14.81 1

Motor Function in α-Synuclein Mice Across Gut Microbiome Treatments — AI Analysis

Removing the gut microbiome (germ-free) nearly eliminates motor deficits in α-synuclein mice: beam traverse time ratio drops from 1.6x to 1.0x vs wild-type

Adding short-chain fatty acids alone restores motor impairment (1.9x slower beam, 2.2x slower pole descent), implicating microbial metabolites as the mechanism

Key Findings

  • Germ-free α-synuclein mice performed nearly identically to wild-type controls across all 4 motor tests, with beam time of 5.3s vs 5.2s
  • SPF (normal microbiome) ASO mice took 65% longer to cross the beam (7.9s vs 4.8s) and 99% longer to descend the pole (12.3s vs 6.2s)
  • Antibiotic treatment protected ASO mice similarly to germ-free conditions: pole descent was just 6.6s vs 6.4s for WT
  • Re-colonizing germ-free mice (ExGF) partially restored deficits: adhesive removal jumped to 10.2s in ASO vs 2.6s in WT — the largest gap of any test
  • Hindlimb clasping scores followed the same pattern: SPF ASO mice scored 1.4/3 vs 0.17 for WT, while germ-free ASO scored just 0.5

Visualizations

Adhesive Removal Time by Treatment
Motor Deficit Ratio (ASO ÷ Wild-Type)
Pole Descent Time by Treatment
Beam Traverse Time by Treatment

Microbiome metabolites are sufficient

Short-chain fatty acids (SCFAs) alone — without live bacteria — reproduced motor deficits in germ-free ASO mice. SCFA-treated ASO mice were 1.9x slower on the beam and 2.2x slower on the pole than wild-type, matching SPF levels. This suggests microbial metabolites, not the bacteria themselves, drive α-synuclein-mediated motor pathology.

This dataset contains 106 records across 6 fields: Treatment, Genotype, Avg Beam cross time (sec), Avg Pole descent time (sec), Avg Adhesive removal time (sec), Hindlimb score.

106 rows · 6 columns · 2026-03-24

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Motor Function Synuclein Mice Across Gut Microbiome Treatments

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